5 Amino 1mq

5-Amino-1MQ (5-amino-1-methylquinolinium) is a small molecule studied in laboratory research as an inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme involved in cellular energy metabolism and NAD+ pathways. It is supplied as a lyophilized powder for researchers who need a consistent, documented compound for experimental work.

This product is for laboratory research use only. It is not for human or veterinary use, and it is not a drug, food, supplement, or cosmetic. Buyers must be 21 or older.

Orders ship on business days. Place your order by 5 PM PT on a business day and it’s picked up by FedEx the same day. Orders placed after the cut-off ship the next business day.

Highlights

• Batch-specific certificate of analysis from Mz Lab
• Lyophilized for stability in transit and storage
• Discreet, protective packaging

Specifications

Product Details

Overview

5-Amino-1MQ is a membrane-permeable small molecule designed to inhibit NNMT. NNMT transfers a methyl group from S-adenosylmethionine (SAM) to nicotinamide, producing 1-methylnicotinamide. Because this reaction touches both the methyl-donor pool and nicotinamide availability, NNMT is an active area of metabolic research.

Mechanism of Interest

NNMT is expressed in tissues including adipose tissue and liver. Published preclinical work has examined how inhibiting NNMT affects cellular NAD+ levels, SAM availability, and energy-related signaling in cell and animal models. Researchers use inhibitors like 5-Amino-1MQ to probe these pathways in controlled experimental settings.

Published Research Context

Early preclinical studies have linked nicotinamide N-methyltransferase (NNMT) to adipose tissue metabolism. In diet-induced obese mice, reducing NNMT expression in fat and liver with antisense oligonucleotides was associated with increased energy expenditure and protection against diet-induced obesity (Kraus et al., 2014). A later study reported that selective, membrane-permeable small-molecule NNMT inhibitors reduced lipogenesis in cultured adipocytes and decreased body weight and white adipose tissue mass in mice fed a high-fat diet (Neelakantan et al., 2018). These findings are preclinical. They were not conducted in humans and do not establish safety or effectiveness for any use in people.

References

1. Kraus D, Yang Q, Kong D, et al. Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity. Nature. 2014;508(7495):258–262. doi:10.1038/nature13198
2. Neelakantan H, Vance V, Wetzel MD, et al. Selective and membrane-permeable small molecule inhibitors of nicotinamide N-methyltransferase reverse high fat diet-induced obesity in mice. Biochemical Pharmacology. 2018;147:141–152. doi:10.1016/j.bcp.2017.11.007

• Neelakantan 2018 is confirmed: the authors, volume, pages and DOI all match the publication record, and the study did test cultured adipocytes and diet-induced obese mice.
• Kraus 2014 is only partly confirmed: search results match its PubMed ID (24717514), but the article pages were blocked, so I took the volume, pages and DOI from what I know of the paper. Open the DOI link once before publishing to confirm them.
• Wording change: Kraus used gene knockdown rather than a drug inhibitor, so the paragraph says “reducing NNMT expression” for that study instead of calling it inhibition.

Purity and Quality Testing

Each batch is tested for identity and purity, and results are provided on the certificate of analysis confirm methods: HPLC, MS. Compare the batch number on your vial label with the COA to confirm you are looking at the right document.

Form and Packaging

The compound is supplied lyophilized in a sealed glass vial with a rubber stopper and cap. Lyophilization removes water, which improves stability during shipping and storage.

Solubility and Preparation

Solubility depends on the salt form and the solvent. Confirm the recommended solvent and solubility from your COA or technical data sheet, and follow your own validated protocol. For aqueous work, sterile diluent such as our Reconstitution Water 3ml may be used where appropriate.

Storage and Handling

Store unopened vials in a cool, dry place, protected from light. Once reconstituted, follow your laboratory’s stability guidelines and avoid repeated freeze-thaw cycles. Discard vials with damaged seals, discoloration, or signs of moisture ingress.

Shelf Life

Unopened, correctly stored vials remain stable until the expiry or retest date on the label

Additional Information

What Is NNMT?

NNMT (nicotinamide N-methyltransferase) is a cytosolic enzyme that methylates nicotinamide, a form of vitamin B3. It consumes SAM, a central methyl donor, and produces 1-methylnicotinamide. Researchers study NNMT in metabolism, aging, and disease models because it sits at the intersection of methylation and NAD+ biology.

Why Researchers Use Small-Molecule Inhibitors

Selective inhibitors let researchers reduce the activity of a single enzyme without altering its gene, which is useful for testing how a pathway behaves in cells or animal models. A well-characterized inhibitor with documented purity helps keep experiments reproducible.

Research Handling Note

Weigh and handle the powder in a controlled environment, using appropriate personal protective equipment. Record the batch number, the date of reconstitution, and the exact solvent and volume used so that your results stay traceable. Reviewed by Bruno M.